Characterization of the central carbon metabolism of Streptococcus pneumoniae by isotopologue profiling
نویسندگان
چکیده
‡Department Genetics of Microorganisms, Interfaculty Institute for Genetics and Functional Genomics, Ernst Moritz Arndt Universität Greifswald, Friedrich-Ludwig-Jahn-Strasse 15a, D-17487 Greifswald, Germany §Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany ¶ Institute of Pharmacy, Ernst Moritz Arndt University Greifswald, Friedrich-Ludwig-JahnStrasse 17, D-17487 Greifswald, Germany
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Characterization of central carbon metabolism of Streptococcus pneumoniae by isotopologue profiling.
The metabolism of Streptococcus pneumoniae was studied by isotopologue profiling after bacterial cultivation in chemically defined medium supplemented with [U-(13)C(6)]- or [1,2-(13)C(2)]glucose. GC/MS analysis of protein-derived amino acids showed lack of (13)C label in amino acids that were also essential for pneumococcal growth. Ala, Ser, Asp, and Thr displayed high (13)C enrichments, wherea...
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